Adipose-derived stem cells (ASCs) are adult stem cells that have increasingly been the subject of studies aimed at therapeutic regeneration due to their ability to differentiate into mesenchymal-lineage cells, undergo self-renewal, and modulate inflammatory states. Previous work has determined that there are sex differences between adipose-derived stem cells in regards to pro- and anti-inflammatory cytokines released during differentiation and under stress conditions, potentially because of differences in gene expression. However, there is still a need for a variety of data on the differences between male and female adipose-derived stem cells, especially under a common activator, lipopolysaccharide (LPS). ASCs derived from male and female adult mice (mASCs) will be treated with acute doses of LPS, alongside their controls. Growth factor and anti-inflammatory cytokine expression will be measured and compared, along with differentiation potential and colony formation. It is hypothesized that there will be a significant difference between male and female mASCs in regard to differentiation ability, growth, and expression of cytokines. This study found significant differences in male and female mASCs’ ability to differentiate and remain healthy and viable under an acute inflammatory stimulus.